Carcinogenesis, Teratogenesis & Mutagenesis ›› 2002, Vol. 14 ›› Issue (3): 167-170.doi: 10.3969/j.issn.1004-616x.2002.03.008

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ANALYSIS OF CYTOCHROME P450 1A COMPOSITION IN HUMAN HEPATOCYTES INDUCED BY BκF

YANG Hui1, LIU Ning2, Laurance S.Kaminsky3   

  1. 1. Department of Immunology, Jiangxi Medical College, Nanchang 330006, China; 2. Department of,Wadsworth Center, Albany , New York 12201, USA
  • Received:2001-07-10 Revised:2001-12-30 Online:2002-07-30 Published:2002-07-30
  • Contact: YANG Hui1

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Abstract: Purpose : To explore the P450 (CYP) CYP1A subfamily composition in the f resh human hepatocyte cultures induced by benzo[κ] fluoranthene (BκF) . Methods : An immunoblot system with Bis2Tris2HCl buffered polyacrylamide gel which clearly resolves human CYP1A1 and CYP1A2 , polyclonal goat anti2human CYP1A1/ CYP1A2 and rabbit anti2human CYP1A2 antibodies were used to probe the expressed CYP1A1 and CYP1Acomposition in six individual human hepatocyte cultures induced with 5μmol/ L (BκF) for twenty2four hours. Results : CYP1A1 was found in five of the six cultures. Both CYP1A1 and 1A2 were simultaneously found in one of them at a ratio of CYP1A1/ CYP1A2 of 0. 942. There was neither CYP1A1 nor CYP1A2 in vehiclet reated hepatocyte cultures. Conclusion : The CYP1A expression in the f resh human hepatocyte cultures induced by BκF is individually variable and it s CYP1A level is different f rom that of human liver microsomal preparations.

Key words: cytochrome P450 1A1, cytochrome P450 1A2, human hepatocyte, polycyclic aromatic hydrocarbon

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